The present study was to evaluate in vivo cutaneous wound healing activity of hydromethanolic extract of M. jalapa radix and to validate its traditional use as medicine for wound healing. In excision wound model, the wound area was measured on the day of infliction (zero day) and subsequently at 4 days interval until the completion of wound healing. In dead space wound model, on 10th post wounding day, the granulation tissues were harvested for estimation of blotting of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (FGF 2) and collagen type III (COL 3A), hydroxyproline content and histological studies. A significant increase (p < 0.05) in rate of wound contraction, decrease in Colony Forming Unit (CFU) count and decrease in epithelisation period was observed in 5 and 10 % treated groups as compared to control. A significant increase (p < 0.05) in hydroxyproline content, up- regulated expression of COL 3A was observed in treated groups as compared to control which was supported by histological evidences. Increase in rate of wound contraction, hydroxyproline content and COL 3A, decrease in epithelisation period and CFUs count shows the wound healing activity of M.jalapa and validate its traditional use in wound and inflammation.